Facts-enriched characterization of cell populations. Morphological information and facts from photos can incorporate on the richness of flow cytometry knowledge. By way of example, the figure displays an usually regular apoptosis assay making use of Annexin V and PI, incorporating mobile imaging to characterize cells in Every single inhabitants to reveal morphologically distinctive characteristics. These insights couldn't have been received from multiplex staining by yourself.
As samples are acquired on the Attune CytPix Flow Cytometer, the significant-speed brightfield digital camera captures and merchants illustrations or photos of detected events in a level up to six,000 illustrations or photos for every next, according to the flow charge and impression dimensions. For greater versatility, Attune Cytometric Program helps you to change the image seize frequency as essential and select a selected gate for picture seize, if needed.
Incorporate acoustic concentrating technologies with high-parameter Investigation for versatile spectral unmixing and traditional workflows.
Uncover new insights into your experimental data by visualizing populations employing put together fluorescence and morphology facts. Our application interface wizards enable downsampling, concatenation (merged files) and dimensionality applications which might be constructed in to the software for an intuitive, streamlined details analysis encounter.
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Right here, a highly trained user has gated singlets confidently. Following assessing the guide singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate consists of in excess of 4% aggregates.
Pair your Attune flow cytometer with the autosampler for improved workflow performance, and increase robotic integration for max throughput.
Our researchers will style panels for you as often times you need—free of charge, no product buy desired.
The Attune NxT cytometer will be the reputable workhorse instrument your lab can depend on for https://trentonrdu80.tinyblogging.com/flow-cytometer-a-revolutionary-tool-for-cell-analysis-77383633 steady and trusted functionality. The Attune CytPix design features a higher-velocity brightfield camera and AI-driven graphic Assessment computer software, with latest upgrades like device Studying instruments and an intuitive consumer interface, enabling enhanced data precision, precision, and speedier insights into sample biology.
Perhaps most of all, these occasions contain cells of Evidently unique phenotypes which could lead to incorrect conclusions relating to double constructive events (particularly in exceptional populations).
Audio waves permit precise cell alignment, leading to exceptional information good quality and ~10X a lot quicker sample flow fees.
We Beforehand demonstrated the power of imaging Vehicle-T/Ramos cell interactions. Let’s take a look at just the inhabitants of finest fascination, the double good gatherings, To find out more. We can easily now use extended morphological parameters (in this article, circularity and skewness of intensity) to more look at the capabilities of such populations and refine gating on these situations, increasing knowledge robustness.
Built-in dimensionality reduction equipment elevated resolution into populations. Fluorescently gated populations again-gated on to UMAP plot to confirm envisioned populace resolution in lysed entire human blood. Consumer can see occasions from Every cluster for visual confirmation of mobile phenotypes.
Adaptable payment system from Agilent Money Alternatives assist you to receive the significant instrumentation you'll need for instant task, or to grow your lab.
At the guts of every Attune Flow Cytometer lies our groundbreaking acoustic concentrating technological know-how, which makes use of audio waves to meticulously align cells inside the flow cell. This progressive approach surpasses common hydrodynamic strategies, leading to faster processing speeds, Extraordinary knowledge high-quality, and greater sample throughput.
The Attune Xenith cytometer is usually a higher-parameter spectral analyzer, giving both spectral unmixing and standard flow abilities for detailed and versatile mobile Evaluation.
The Attune CytPix Flow Cytometer makes it possible for customers to visualise samples throughout acquisition, validate the accuracy of gating procedures, and assist success with trustworthy documentation for publication uses.
What seemed to be a homogeneous population is definitely additional varied—an Perception that needs to be deemed when interpreting effects.