Details-enriched characterization of cell populations. Morphological details from visuals can increase into the richness of flow cytometry facts. By way of example, the determine displays an if not standard apoptosis assay applying Annexin V and PI, including cell imaging to characterize cells in Just about every population to reveal morphologically distinct attributes. These insights couldn't are already acquired from multiplex staining alone.
Irrespective of whether you’re a newbie or a professional, take a look at our assets to progress your analysis and know-how in flow cytometry.
(B) RAW 264.seven macrophages are sometimes annotated inaccurately with regard to particle rely due to their complexity and noncircular borders. The amount of singlets readily available for analysis improves when the consumer trains and employs a more correct product.
The award-winning Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing using a higher-speed brightfield camera for simultaneous superior throughput flow cytometry and superior resolution brightfield imaging.
*Pricing is indicative only and is also subject matter to alter. You should Make contact with your Agilent agent with any inquiries. The information established forth herein is not really a proposal or a dedication, both equally of which might be subject to credit history acceptance, stipulations, and availability with Agilent. Agilent Economical Solutions is offered in picked nations around the world.
Furthermore, it is actually illuminating to find out how many doublets slip through regular singlet gates, and fantastic to validate the status of unexpected double-beneficial gatherings in my Investigation."
Pair your Attune flow cytometer using an autosampler for elevated workflow efficiency, and add robotic integration for max throughput.
Steady graphic top quality even at large throughput Acoustic focusing and also a substantial-pace digital camera Incorporate to graphic these T cells regularly at reduced or superior flow costs. Effortlessly alter emphasis and digital camera settings to fulfill experimental necessities.
Early indications of this contamination had originally been dismissed as debris. Backgating to discover new populations. Backgating imaged cells within the Attune CytPix Flow Cytometer also lets you use morphological characteristics to find attention-grabbing subpopulations that could not be clear from flow more info cytometry info by itself.
Consumers can look at party images in true-time to substantiate sample good quality, improve protocols, and record the morphology of every cell inhabitants identified for analysis. Imaging seize also benefits from acoustic focusing, which really helps to position the cells making sure that a pointy, centered graphic is received.
Sound waves empower exact cell alignment, causing Excellent details quality and ~10X more rapidly sample flow premiums.
Application Notice: Automated picture Evaluation reduces person-to-user variability in flow cytometry gating procedures
Constructed-in dimensionality reduction equipment increased resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to confirm anticipated populace resolution in lysed entire human blood. User can view activities from Every single cluster for Visible confirmation of cell phenotypes.
Adaptable payment approach from Agilent Economical Remedies help you obtain the essential instrumentation you will need for instant project, or to develop your lab.
At the guts of each Attune Flow Cytometer lies our revolutionary acoustic focusing technologies, which utilizes audio waves to meticulously align cells in the flow cell. This impressive method surpasses common hydrodynamic techniques, resulting in quicker processing speeds, Remarkable info high-quality, and increased sample throughput.
As the mobile line experienced Formerly been analyzed over the Attune CytPix Flow Cytometer, the scientists went back again to the images and have been capable to doc the microbial an infection a minimum of five days previously.
The Attune CytPix Flow Cytometer will allow buyers to visualize samples in the course of acquisition, validate the precision of gating tactics, and support outcomes with reliable documentation for publication uses.
Right here, we demonstrate that by gating methods using impression-dependent quantitative parameters can distinguish interacting cells from coincident activities to a lot more precisely examine interacting cells.